Response of Flavonoids Production to Some Elicitors in Callus and Cell Suspension Culture of Ginkgo (Ginkgo biloba L.)

Aims: This study was conducted to investigate how maximize the production of flavonoids from ginkgo leaves callus or cell suspension cultures. Study Design: The experiment implemented in a completely randomized design with 3 replicates. Place and Duration Study: carried out at Research Laboratory Tissue Culture Preservation Germplasm, Horticulture Institute, Agric. Res. Center, Egypt 2020 2022. Methodology: Adenine sulfate pyruvic acid were added concentrations 0.1, 0.2, 0.3 mg/l, for each. Cadmium lead acetate (2 4 mg/l each) suspension. Jasmonic (15, 50, 100 µM), AgNO3 (25, 75 µM) tryptophan (50, 100, 200 mg/l) used hard culture. Results: Results indicated that maximum fresh weight (6.50 g) obtained (0.5 grown on MS medium supplemented adenine 0.2 while 2.0 increased dry (0.89g) after second subculture. packed volume (PCV) (24.33%) cycle growth. best flavonoid (91.29 mg/100 g DW) produced growth treated 25 µM. Treating Ginkgo biloba cultured jasmonic µM inhibition AChE serum AD patients 235.2 U/l (untreated serum) 76.08 38.71 ug/ml compared ® 260 mg which inhibit activity 55.73 26.00 amount 25.00 ul all. Conclusion: is valuable source anticholinesterase agents could be pharmaceutical preparations by using culture technique AgNO3.